Generator

Part:BBa_K2310004

Designed by: Yulong Han   Group: iGEM17_XMU-China   (2017-10-18)


Double-repoter (LacZ-alpha and RFP) generator with T7 promoter

This part consists of the T7 promoter and double-repoter (LacZ-alpha and RFP) generator. The subpart BBa_I732092 has two reporters, so that the activity of upstream promoter can be qualitatively observed on a X-gal plate, or be quantificationally measured using ONPG assay or fluorescent assay.

T--XMU-China--Resultsimg6.png

Figure. BBa_K2310002, BBa_K2310003, BBa_K2310004 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 887
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None